Endocrine Abstracts

Objective: Identify predictors of pulsatile GH secretion following a triple secretagogue stimulus (TSS, Ghrelin and GHRH preceded by an infusion of Arginine) in a cohort of healthy postmenopausal women and healthy older men.Hypothesis: Even in older subjects, total GH secretory capacity is gender dependent.Design: 23 men and 19 women participated in 6 separate, overnight 16-h infusion sessions at Clinical Research Center. At the en...

Glucocorticoids (Gcs) act via the intracellular glucocorticoid receptor (GR) to regulate cellular homeostasis. GR is a ligand-activated transcription factor that mediates effects within the nucleus or cytoplasm to regulate genomic and non-genomic events. Localisation of the GR to a specific cellular compartment is therefore an important determinant of the cellular response to Gcs.Live cell imaging of GR trafficking using fluorophore-tagged GR shows drama...

Glucocorticoids (Gcs) act via the intracellular glucocorticoid receptor (GR) to regulate cellular homeostasis. Previous studies have demonstrated altered Gc sensitivity during mitosis. This reduced sensitivity can be explained only in part through the selective export of GR in mitotic cell populations. In this study ligand-independent, cell cycle-dependent effects on GR have been explored.Mitotic cells show a marked increase in ligand-independent ser211 ...